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	Provedor de dados BABT (3) Acta Botanica (1) PAB (1) Autor Steiner,Neusa (5) Guerra,Miguel Pedro (4) Santos,André Luis Wendt dos (2) Silveira,Vanildo (2) Cangahuala-Inocente,Gabriela Claudia (1) Demeda,Camila Luisa Bernhardt (1) Maldonado,Sara (1) Maldonado,Sara Beatriz (1) Maraschin,Marcelo (1) Seger,Guilherme Dubal dos Santos (1) Trevisan,Rafael (1) Vidor,Mário (1) Vieira,Felipe do Nascimento (1) Mais... Palavra-chave Somatic embryogenesis (3) Aquatic macrophyte (1) Araucaria angustifolia (1) Araucariaceae (1) Carbohydrate (1) Cell competence (1) Coastal lake (1) Conifer (1) Conifers (1) Cyperaceae (1) Eleocharis (1) Embryo maturation (1) Germinability (1) Glucose (1) Metabolism (1) PH changes (1) Picea abies (1) Pineapple guava (1) Pinus sp. (1) Protein (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Ano 2018 (1) 2010 (1) 2009 (1) 2005 (1) 2002 (1) País Brazil (5) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Biochemical and morphological changes during the growth kinetics of Araucaria angustifolia suspension cultures BABT Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Maraschin,Marcelo; Guerra,Miguel Pedro. Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 µM 2,4-dichlorophenoxyacetic acid, 1 µM 6-benzylaminopurine and 1 µM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive reduction during culture time. For both the embryogenic cultures a preferential uptake of glucose in the late stages of cell growth kinetics was observed. The extracellular protein content was similar for both the embryogenic cultures. Acetocarmine and Evan's blue double stain showed major differences for early somatic embryo organisation, in which only the embryogenic... Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucariaceae; Glucose; PH changes; Protein; Somatic embryogenesis. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300001 Effect of carbon source on morphology and histodifferentiation of Araucaria angustifolia embryogenic cultures BABT Steiner,Neusa; Vieira,Felipe do Nascimento; Maldonado,Sara; Guerra,Miguel Pedro. The aim of the present work was to establish in vitro conditions for the induction, stabilization and proliferation of embryogenic cultures of A. angustifolia. Pre-cotyledonary staged zygotic embryos inoculated BM medium supplemented with 5 µM 2,4- D, 2 µM BAP and Kin, and 3% maltose or sucrose resulted in 66.7% induction rate. The rate of induction of embryogenic cultures was affected by the carbon source, as well the multiplication and morphology of the embryogenic cultures. Embryogenic cultures maintained in BM medium with maltose presented bipolar morphology. Globular somatic embryos were obtained BM medium with 9% (PEG) and (9%) maltose. These results could establish an in vitro regenerative protocol towards the conservation and improvement of this... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell competence; Somatic embryogenesis; Carbohydrate; Conifers. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800005 Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana PAB Cangahuala-Inocente,Gabriela Claudia; Steiner,Neusa; Maldonado,Sara Beatriz; Guerra,Miguel Pedro. The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid-Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Total sugars; Starch; Pineapple guava; Metabolism. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000300001 Reproductive phenology and germination of Eleocharis laeviglumis R. Trevis. & Boldrini (Cyperaceae) Acta Botanica Demeda,Camila Luisa Bernhardt; Seger,Guilherme Dubal dos Santos; Steiner,Neusa; Trevisan,Rafael. ABSTRACT Aquatic macrophytes are important components of freshwater ecosystems, of which Cyperaceae is the most diverse family. Many species of Cyperaceae form dense populations along lake margins of the southern coast of Brazil, but little is known about their sexual reproductive strategies. We characterized the reproductive life cycle of Eleocharis laeviglumis, an abundant emergent macrophyte of coastal wetlands in southern Brazil, by assessing its reproductive phenophases and estimating the number of its flowers and potentially viable fruits per inflorescence in a natural population. We also tested seed germinability and vigor for a period of four months during a single reproductive season. The species possesses dichogamous and protogynous spikelets... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aquatic macrophyte; Coastal lake; Cyperaceae; Eleocharis; Sexual reproduction; Spikelet; Germinability; Reproductive phenology; Seed vigor. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000300487 Somatic Embryogenesis in Parana Pine (Araucaria angustifolia (Bert.) O. Kuntze) BABT Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Vidor,Mário; Guerra,Miguel Pedro. Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Araucaria angustifolia; Conifer; Somatic embryogenesis; Picea abies; Embryo maturation; Pinus sp.. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015 Registros recuperados: 5 Primeira ... 1 ... Última

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